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- W2363098462 abstract "Isolation of high quality RNA is an essential prerequisite for gene expression analysis on rape seed development mechanism.The secondary dormant seeds of mature rapeseed were rich in polysaccharids and polyphenols,while the old tissues had high Rnase activity and low physiological activity.According to these characteristics,we developed a rapid and simple method for the isolation of total RNA through choosing RNA plant plus reagent DP437(TIANGEN) and taking some measures such as adding β-mercaptoethanol,NaAc and reducing the extraction times to inhibit RNA enzyme activity,preventing oxidation of phenolic compounds and removing the effects of polysaccharide substance respectively.Two clear bands of 28S rRNA and 18S rRNA isolated from rapeseeds could be detected by agarose gel electrophoresis.RNA extracted by this method was intact without degradation.The D260 nm/D280 nm absorbance ratio was 1.92-2.05.These results showed that the RNA obtained from rapeseed with this method had high quality and were suitable for the subsequent molecular studies,including mRNA separation,standard mRNA library constructing and high-throughput RNA-seg etc.At the same time this method would provide technical guarantee for exploring the molecular mechanism of secondary dormancy in rapeseed,and also could be used for isolation of total RNA from mature dormant seeds rich in polysaccharides and polyphenols of other plants." @default.
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- W2363098462 date "2013-01-01" @default.
- W2363098462 modified "2023-09-24" @default.
- W2363098462 title "A fast and efficient method for extraction of RNA from secondary dormant seeds of mature rapeseed." @default.
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