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- W2363103687 abstract "Two R-phycoerythrins(R-PE),PE272-1 and PE377-2,are isolated and purified from the marine red alga Polysiphonia urceolata by the ion exchange chromatography on DEAE-52 and by the gel filtration chromatography on Sephadex G-150 and Sephacryl S-300.The two prepared R-PEs have very similar spectral properties and show single band both in native PAGE and in native isoelectric focusing(IEF).Isoelectric points(pIs) of PE272-1 and PE377-2 are 4.5 and 4.4,respectively.The polypeptide analysis by SDS-PAGE and denaturing IEF demonstrates that PE272-1 and PE377-2 contain the same colored subunits 17.5 kD α,19.5kD β and 34.4kD γ,and that α and β show pI 5.7 and 5.4,respectively.But PE377-2 also has other two colorless polypeptids and of 57.9kD and 60.3 kD,which PE272-1 has not.The three subunit proportions of PE272-1and PE377-2,3α∶3β∶γ1,indicate that PE272-1 and PE377-2 mainly exist in hexameric forms of(αβ)3-γ-(αβ) 3-γ and LR-(αβ)3-γ-(αβ)3-γ.These results give worthwhile experiment data for the investigation on the rod structure domain of R-PE constructing in phycobilisomes of red macroalgae." @default.
- W2363103687 created "2016-06-24" @default.
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- W2363103687 date "2011-01-01" @default.
- W2363103687 modified "2023-09-23" @default.
- W2363103687 title "Two R-phycoerythrins from a Marine Red Alga Polysiphonia urceolata Grev" @default.
- W2363103687 hasPublicationYear "2011" @default.
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