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- W2363109867 abstract "Hydrolysis of the earthworm with subtilisin from Bacillus subtilis AS1.398 and self-enzyme of earthworm,the amino acid components and distribution of the relative molecular weight of earthworm enzymatic products were studied.The optimum conditions of enzymatic hydrolysis were determined through mono-factor analysis and orthogonal test(L9(34)) using the content of amino acid nitrogen and polypeptide concentration of earthworm as testing indexes.The optimum conditions of earthworm hydrolyzed by AS1.398 subtilisin from Bacillus subtilis were pH 6.5,temperature 50 ℃,time 8 h,and 1% enzyme concentration.Under these conditions,the content of amino acid nitrogen and polypeptide concentration of earthworm enzymatic hydrolysates can reach 16.55 mmol/100 mL and 9.22 mg/mL.The optimum conditions of autolyzed earthworm were pH 7.0,substrate concentration 1∶2,temperature 50 ℃,time 6 h.Under these conditions,the content of amino acid nitrogen and polypeptide concentration of earthworm enzymatic hydrolysates went up to 21.55 mmol/100 mL and 11.65 mg/mL.And the two enzymatic hydrolysates of earthworm mainly consisted of polypeptide,small peptide and amino acids with molecular weight below 5000,and about 80% of those with molecular weight less than 300.They have plenty of essential amino acids,and balanced composition of amino acids which may benefit the manufacture of new additives of amino acid microelement and small peptide.Through controlling enzymatic hydrolysis,earthworm can avail itself of the enzyme instead of exogenous enzymes,which can reduce the cost." @default.
- W2363109867 created "2016-06-24" @default.
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- W2363109867 date "2007-01-01" @default.
- W2363109867 modified "2023-09-28" @default.
- W2363109867 title "Enzymatic Technology and Analysis of Enzymatic Products of Earthworm with Subtilisin from Bacillus subtilis AS1.398" @default.
- W2363109867 hasPublicationYear "2007" @default.
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