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- W2363125497 abstract "Objective To prepare monoclonal antibodies against Brucella abortus and to identify its partial characteristics.Methods BALB/c mice were immunized with S85A(local strain of Brucella abortus type Ⅲ),then spleen cells from immunized mice and SP2/O myeloma cells were fused by PEG.Hybridoma supernatants were screened by indirect ELISA and hybridoma cell strains secreting monoclonal antibody(McAb) against B.abortus were established.Results By 3 times limiting dilution,4 hybridoma cell strains secreting monoclonal antibody(McAb) against B.abortus were established.The immunoglobulin subclass of the 4 McAbs were IgG2b(3) and IgG3(1),respectively.Specificity of the 4 McAbs was analyzed with indirect ELISA.Conclusions Indirect ELISA indicates that the McAbs only reacts to B.abortus,but not to the other species of brucellas.It suggests the established cell strains are hybridoma cell strains secreting monoclonal antibody(McAb) against B.abortus." @default.
- W2363125497 created "2016-06-24" @default.
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- W2363125497 date "2008-01-01" @default.
- W2363125497 modified "2023-09-28" @default.
- W2363125497 title "Preparation and Identification of Partial Characteristics of Monoclonal Antibodies Against Brucella abortus" @default.
- W2363125497 hasPublicationYear "2008" @default.
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