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- W2363126246 abstract "Objective:To establish a new method for detecting the expression of nerve growth factor messenger ribonucleic acid(NGF mRNA) in cerebrum tissue of rats.Methods:Total RNA was isolated from cerebrum tissue by Trizol-one-step method.Specific oligonucleotide of NGF mRNA gene fragments were amplificated by RT-PCR.The method of detection of NGF mRNA expression using SYBR Green Ⅰ real time RT-PCR was constructed.The specificity of the method was confirmed by melting curve.Housekeeping gene β-actin was applied to rectify the quantity of NGF.Results:SYBR Green real-time PCR was developed with high specificity and sensitivity for detecting of rat NGF mRNA.Conclusions:The method of detection of NGF mRNA expression using real time RT-PCR is constructed successfully." @default.
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- W2363126246 date "2012-01-01" @default.
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- W2363126246 title "Establishment of fluorescent quantitative real time RT-PCR method for detection of the expression of nerve growth factor mRNA" @default.
- W2363126246 hasPublicationYear "2012" @default.
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