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- W2363130494 abstract "【Objective】 To explore the neural differentiation potential of a new human embryonic stem cell line,SYSU-7,through embryoid body formation under different culture condition.【Methods】 Detail characterization of SYSU-7 cells were carried out by detecting the expression of Oct4,SSEA4,TRA-1-60,AKP,and multi-lineage differentiation capacity in vivo and in vitro.20% KSR medium(KSR) and neural progenitor medium(NPM) were used to induce neural differentiation of SYSU-7 cells,respectively.Embryoid bodies(EBs) were generated and cultured in suspension for 4d or 7d before attached to polyornithine and fibronectin(PO+FN) coated culture dishes.Neural stem/progenitor cells derived from SYSU-7 cells were identified by the immunocytochemical assays.【Results】 The immunostaining analysis showed that SYSU-7 cells expressed the undifferentiated marker Oct4,SSEA4,and TRA-1-60,as well as the AKP.The results also demonstrated that SYSU-7 cells have the capacity to differentiate into three germ layers both in vitro and in vivo.KSR medium was found to be superior to NPM for neural differentiation of SYSU-7 cells.EBs cultured in suspension for 7 d resulted in earlier appearance and greater amount of rosette structures than 4-day EBs.【Conclusion】 SYSU-7 cells possessed the distinct characteristics of human embryonic stem cells,self-renew and pluripotency.The results provided a new cell model and may have implications for neural differentiation studies." @default.
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- W2363130494 date "2011-09-20" @default.
- W2363130494 modified "2023-09-22" @default.
- W2363130494 title "Neural Differentiation of Human Embryonic Stem Cells" @default.
- W2363130494 hasPublicationYear "2011" @default.
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