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- W2363134327 abstract "By comparing the effect of pig oviduct epithelium cells derived from different estrous cycle for culturing early transgenic mouse embryos,an effective co-culture system for culture of early embryos in vitro was built.The retention of foreign gene in mouse embryos was detected by PCR further.The results showed that there was no significant difference between the blastocyst rate of embryos co-cultured with pig oviductal epithelium cells(whether or not primary and passage cells) derived from pig oviduct during same estrous cycle.Blastocyst rate of embryos co-cultured with pig oviduct epithelium cells from pig oviduct during diestrus was significantly lower than metestrous.The rate of transgenic positive embryos in 2-cell,4-cell,8-cell and blastocyst stage was 96%,63%,43%,and 19%,respectively.All in all,pig oviductal epithelium cells derived from pig oviduct during metestrous can improve the development of mouse early embryos when mouse early embryos were co-cultured with it.With development of early embryos microinjected with foreign gene,the rate of transgenic positive embryos decreased gradually." @default.
- W2363134327 created "2016-06-24" @default.
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- W2363134327 date "2007-01-01" @default.
- W2363134327 modified "2023-09-23" @default.
- W2363134327 title "Effect of swine oviduct epithelium cells on development of co-cultured mouse early embryos in vitro" @default.
- W2363134327 hasPublicationYear "2007" @default.
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