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- W2363215087 abstract "Objective To observe the apoptosis effect of hydrazine on cardiac fibroblasts(CFB) cultured in vitro, so as to investigate its mechanism. Methods Specimens of cardiac fibroblasts cul- tured from heart tissue of neonatal rats were divided into control group and hydrazine group. The cul- tured fibroblasts of hydrazine group were treated with 2 mmol/L hydrazine for 72 h. The cell nucleus changes of apoptosis were examined by fluorescence microscope using Hoechst33258 stain. The rates of apoptosis and necrosis of cardiac fibroblasts stained by Annexin V-FITC/Propidiun Iodide Apoptosis Detect Kit I were detected by flowcytometry. The mitochondria membrane potential of cells stained by rhodamine123 was assayed by flowcytometry. The expression of Caspase-3, Bax, Bcl-2 were detected by Western-blot. Results The nucleus of apoptosis cardiac fibroblasts showed nucleus aggregation phenomenon. As compared with control group, the rates of apoptosis and necrosis in hydrazine treated group were significantly higher, while the mitochondria membrane potential of cells was significantly lower, the expression of Caspase-3 and the Bax expression were increased, while the Bcl-2 expression decreased. Conclusions Hydrazine can induce CFB apoptosis. The possible mechanism is that hy- drazine leads injury of mitochondria membrane and decrease of the mitochondria membrane potential, which in turn leads opening of transition pore,and release of cytochrome C into cytosol interacting with apoptotic protease-activating factor-1(Apaf-1), ATP/dATP, and pro-Caspase-9 forming apoptosome, and then activating pro-Caspase-3, results in CFB apoptosis." @default.
- W2363215087 created "2016-06-24" @default.
- W2363215087 creator A5023090667 @default.
- W2363215087 date "2006-01-01" @default.
- W2363215087 modified "2023-09-23" @default.
- W2363215087 title "Experimental study on mechanism of the apoptosis of cardiac fibroblasts induced by hydrazine" @default.
- W2363215087 hasPublicationYear "2006" @default.
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