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- W2363243336 abstract "Objective To observe apoptosis induced by low-dose aminophylline in healthy and asthmatic human peripheral blood CD4+CD25+T regulatory cells(Treg).Methods Peripheral CD4+CD25+Treg of healthy and bronchial asthma(asthma)was separated with Percoll(1.073 g/ml)and harvested by filtration through nylon column and were separated after magnetic separation.Then,cells were cultured for 72 h while divided into low dose aminophylline(1.13 mg/L)and blank groups.The incidence rate of apoptosis was analyzed by flow cytometry.Results ①The separated rates of CD4+CD25+Treg were 77.4%～92.3% in healthy group,and were 75.2%～93.8% in asthmatic group.②The ratio of CD4+CD25+Treg in CD4+T cells was 4.12%～7.98% in health adults,and 4.51%～8.68% in asthma.There were no significant statistic differences between them.③Low dose aminophylline could upregulate the apoptosis ratios on CD4+CD25+Treg in health and asthma(P0.05).Conclusions Low-dose aminophylline(1.13 mg/L)could educe immunomodulatory effect by inducing apoptosis in peripheral blood CD4+CD25+ Treg." @default.
- W2363243336 created "2016-06-24" @default.
- W2363243336 creator A5046864915 @default.
- W2363243336 date "2009-01-01" @default.
- W2363243336 modified "2023-09-25" @default.
- W2363243336 title "Apoptosis induced by low-dose aminophylline in asthmatic peripheral blood CD4~+CD25~+ T regulatory cells" @default.
- W2363243336 hasPublicationYear "2009" @default.
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