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- W2363284555 abstract "The coding sequence of rice preferable codon optimized cry2A* gene was amplified via polymerase chain reaction (PCR) from recombinant plasmid pUC18-3Z/Cry2A*. Then the PCR products of cry2A* gene were inserted into expression vector pET-28a(+) using restriction endonucleases Nde I and BamH I, resulting in the recombinant expression plasmid pET-28a(+)/ Cry2A* expressing Cry2A* proteins with only 6 His-tags attached to its N-terminus. Subsequently, the expression vector pET-28a(+)/Cry2A* was introduced into E. coli BL21 (DE3). The Cry2A* protein was expressed mainly in soluble form in the presence of isopropyl-β-D-thiogalactopyranoside (IPTG) with final concentration 0.05 mmol/L by inducing for 3 h at 20 ℃. The recombinant protein was purified by Ni-NTA affinity chromatography, and the purity is up to 95% according to thin layer scanning analysis." @default.
- W2363284555 created "2016-06-24" @default.
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- W2363284555 date "2008-01-01" @default.
- W2363284555 modified "2023-09-26" @default.
- W2363284555 title "Expression of Rice Codon Optimized cry2A* Gene in Escherichia coli and Purification of Its Expressed Proteins" @default.
- W2363284555 hasPublicationYear "2008" @default.
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