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- W2363291269 abstract "Serious epidemic of great yellow croaker(Pseudosciaena crocea) cultured in net-cage in Ningbo and Taizhou,Zhejiang Province,broke out in spring 2004 and 2005.The main symptom of diseased fish was the appearance of many 0.1~1mm size white spots in the spleen and kidney.Strain GYCWS isolated from the diseased fish was confirmed to be the pathogen of the epidemic by challenge test,and its LD50 was 8.5×104 CFU/mL.The morphological,physiological and biochemical characters of the GYCWS colony isolate was then determined.About 1.5Kb fragments of the isolate was amplified by PCR using general primers of 16s ribosomal DNA.The PCR product was extracted and cloned into pMD 18-T vector.After the transformation into E.coli TG1,the recombination plasmid was identified by restriction enzyme digestion and by PCR.One selected clone was then sequenced.The BLAST results of the sequence showed that it has high homology(99%) with respective sequences of Pseudomonas putida.The accession number of 16S rRNA gene sequence was DQ648602 on Genbank.The results indicated that GYCWS isolate belonged to Pseudomonas putida.The test of sensitivities of strain GYCWS to 14 kinds of antibiotics revealed that the pathogen was sensitive to kanamycin,gentamicin,amikacin,norfloxacin,tetracycline and streptomycin." @default.
- W2363291269 created "2016-06-24" @default.
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- W2363291269 date "2008-01-01" @default.
- W2363291269 modified "2023-09-24" @default.
- W2363291269 title "Isolation and identification of pseudomonassis pathogen from cultured Pseudosciaena crocea" @default.
- W2363291269 hasPublicationYear "2008" @default.
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