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- W2363292743 abstract "ObjectiveTo construct a mammalian expression plasmid pcDNA3.1-β amyloid (pcDNA3.1-Aβ1-42), and to lay a foundation for further studying the protective immunity of pcDNA3.1-Aβ1-42 as an AD, sDNA vaccine. MethodsIsolation the genome DNA from Tg2576 mice and amplified by PCR. The gene and the plasmid pcDAN3.1 were digested with Kpn I and Xho I respectively, and the gene was cloned into the pcDNA3.1 directionally. The recombinant vector pcDNA3.1-Aβ1-42 was characterized by restriction enzymes digestion and sequencing identification. ResultsThe destination gene, 126 bp long, was amplified by PCR distinctively and inserted successfully into the plasmid pcDNA3.1. Enzymes digestion and sequencing identification showed that Aβ1-42 recombined with pcDNA3.1 exactly. ConclusionThe mammalian expression vector pcDNA3.1-Aβ1-42 is successfully constructed" @default.
- W2363292743 created "2016-06-24" @default.
- W2363292743 creator A5007559627 @default.
- W2363292743 date "2006-01-01" @default.
- W2363292743 modified "2023-09-26" @default.
- W2363292743 title "Construction and identification of recombinant plasmid pcDNA3-β amyloid" @default.
- W2363292743 hasPublicationYear "2006" @default.
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