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- W2363311704 abstract "Objective To establish a high performance liquid chromatographic (HPLC) method for simultaneous determination of rhein,emodin,aloe-emodin,chryphanol,and physcion in Rheum Officinalis Baill water extract. Methods The Inertsil ODS3 C18 reversed-phase column (250 mm×4.6 mm,5 μm) was adopted as stationary phase. The A (1% acetic acid)-B (methanol) with elution 8︰92 (v/v) was adopted as mobile phase. The flow rate was 1 ml/min. Detection was performed at wavelength of 254 nm under 30℃. Results These components were completely separated within 20 min under this chromatographic condition. The linear range for rhein,emodin,aloe-emodin,chryphanol,and physcion was 5~50,2~12,2~12,2~12,and 2~12 mg/L,respectively. The average recovery (n=3) was 102.89% for rhein (RSD 2.97%),102.22% for emodin (RSD 4.60%),101.87% for aloe-emodin (RSD 3.26%),103.90%,for chryphanol (RSD 1.33%),and 103.69% for physcion (RSD 1.77%).Conclusion This method is simple and quick with high accuracy,and it can be applied to the determination of rhein,emodin,aloe-emodin,chryphanol,and physcion in Rheum officinalis Baill water extract with satisfactory results." @default.
- W2363311704 created "2016-06-24" @default.
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- W2363311704 date "2009-01-01" @default.
- W2363311704 modified "2023-09-23" @default.
- W2363311704 title "Simultaneous Determination of Anthraquinones in Rheum officinalis Baill Water Extract by RP-HPLC" @default.
- W2363311704 hasPublicationYear "2009" @default.
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