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- W2363315654 abstract "To construct short hairpin RNA interfering expression vector of TDRG1,and detect the specific interfering effect of TDRG1-shRNA expression vector on NTERA-2 cells.Oligos for short hairpin RNA targefing for TDRG1 were designed and connected to the expression vector pGPU6/GFP/Neo to construct the TDRG1 shRNA expression vector. The recombinant plasmid TDRG1-shRNA486, TDRG1-shRNA738, TDRG1-shRNA921 and lipofectamine ™2000 were used to generate and transfect shRNA into NTERA-2 cells. Expression of TDRG1 mRNA was assayed by RT-PCR.TDRG1-shRNA expression vector was successfully constructed. TDRG1-shRNA486 was more effective in the suppression of TDRG1 with significant reduction of TDRG1 mRNA.TDRG1-shRNA can interfere the expression of TDRG1 in NTERA-2 cells." @default.
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- W2363315654 date "2012-10-01" @default.
- W2363315654 modified "2023-09-27" @default.
- W2363315654 title "[Construction of TDRG1 shRNA expression vector and interfering effect of TDRG1 shRNA expression vector on NTERA-2 cells]." @default.
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- W2363315654 doi "https://doi.org/10.3969/j.issn.1672-7347.2012.10.002" @default.
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