Matches in SemOpenAlex for { <https://semopenalex.org/work/W2363373035> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2363373035 abstract "In order to further the study of the biological activity and function of ASP from Vibrio alginolyticus,the recombinant plasmid pET23d-asp were transformed into the host E.coli BL21(DE3).The expressed product was purified and analyzed,and the expression conditions were optimized.The soluble ASP was purified when the chromatogram column was eluted by 100 mmol/L imidazole eluting buffer.The purified ASP had enzymatic activity and its relative molecular weight was 42 000.When the expression was induced with 1 mmol/L Isopropyl-β-D-thiogalactopyranoside(IPTG) for 16 h at 28 ℃,the maximum amounts of soluble proteins were obtained." @default.
- W2363373035 created "2016-06-24" @default.
- W2363373035 creator A5032588826 @default.
- W2363373035 date "2008-01-01" @default.
- W2363373035 modified "2023-09-28" @default.
- W2363373035 title "Optimization of Expression Conditions and Activity of asp Gene from Vibrio alginolyticus in Escherichia coli" @default.
- W2363373035 hasPublicationYear "2008" @default.
- W2363373035 type Work @default.
- W2363373035 sameAs 2363373035 @default.
- W2363373035 citedByCount "0" @default.
- W2363373035 crossrefType "journal-article" @default.
- W2363373035 hasAuthorship W2363373035A5032588826 @default.
- W2363373035 hasConcept C104317684 @default.
- W2363373035 hasConcept C153911025 @default.
- W2363373035 hasConcept C170344550 @default.
- W2363373035 hasConcept C185592680 @default.
- W2363373035 hasConcept C22744801 @default.
- W2363373035 hasConcept C2776154503 @default.
- W2363373035 hasConcept C2778968793 @default.
- W2363373035 hasConcept C40767141 @default.
- W2363373035 hasConcept C523546767 @default.
- W2363373035 hasConcept C54355233 @default.
- W2363373035 hasConcept C547475151 @default.
- W2363373035 hasConcept C55493867 @default.
- W2363373035 hasConcept C86803240 @default.
- W2363373035 hasConcept C89423630 @default.
- W2363373035 hasConceptScore W2363373035C104317684 @default.
- W2363373035 hasConceptScore W2363373035C153911025 @default.
- W2363373035 hasConceptScore W2363373035C170344550 @default.
- W2363373035 hasConceptScore W2363373035C185592680 @default.
- W2363373035 hasConceptScore W2363373035C22744801 @default.
- W2363373035 hasConceptScore W2363373035C2776154503 @default.
- W2363373035 hasConceptScore W2363373035C2778968793 @default.
- W2363373035 hasConceptScore W2363373035C40767141 @default.
- W2363373035 hasConceptScore W2363373035C523546767 @default.
- W2363373035 hasConceptScore W2363373035C54355233 @default.
- W2363373035 hasConceptScore W2363373035C547475151 @default.
- W2363373035 hasConceptScore W2363373035C55493867 @default.
- W2363373035 hasConceptScore W2363373035C86803240 @default.
- W2363373035 hasConceptScore W2363373035C89423630 @default.
- W2363373035 hasLocation W23633730351 @default.
- W2363373035 hasOpenAccess W2363373035 @default.
- W2363373035 hasPrimaryLocation W23633730351 @default.
- W2363373035 hasRelatedWork W1417509926 @default.
- W2363373035 hasRelatedWork W1977849537 @default.
- W2363373035 hasRelatedWork W2003547762 @default.
- W2363373035 hasRelatedWork W2166472350 @default.
- W2363373035 hasRelatedWork W2350297908 @default.
- W2363373035 hasRelatedWork W2360924512 @default.
- W2363373035 hasRelatedWork W2369905149 @default.
- W2363373035 hasRelatedWork W2374766412 @default.
- W2363373035 hasRelatedWork W2380208042 @default.
- W2363373035 hasRelatedWork W2384680018 @default.
- W2363373035 hasRelatedWork W2387374218 @default.
- W2363373035 hasRelatedWork W2387430415 @default.
- W2363373035 hasRelatedWork W2387820646 @default.
- W2363373035 hasRelatedWork W2389545618 @default.
- W2363373035 hasRelatedWork W2416938353 @default.
- W2363373035 hasRelatedWork W2431862859 @default.
- W2363373035 hasRelatedWork W2460718027 @default.
- W2363373035 hasRelatedWork W2462253365 @default.
- W2363373035 hasRelatedWork W2678310535 @default.
- W2363373035 hasRelatedWork W3141037400 @default.
- W2363373035 isParatext "false" @default.
- W2363373035 isRetracted "false" @default.
- W2363373035 magId "2363373035" @default.
- W2363373035 workType "article" @default.