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- W2363396415 abstract "To obtain pyrene-degrading bacteria,soil and sludge samples were collected from severely PAHs-contaminated sites in South China.Strains W52 and W74 were isolated by agar plate subliming method.The two strains were identified as Mycobacterium sp.based on morphological observations,physiological test and 16S rDNA sequence analysis.When incubated on solid plates containing a thin pyrene film,W52 and W74 degraded 13.2 μg and 11.8 μg pyrene,respectively,in 23 days.When incubated in a liquid mineral salt medium containing 50 mg·L-1 and 100 mg·L-1 pyrene,W52 degraded 57.8% and 20.5% of the pyrene in 2 days;while W74 degraded 19.2% and 4.0%.Pyrene-degradation rates increased with incubation time.On the 10th day,W52 pyrene-degrading rates reached 96.3% and 82.3% respectively,while W74 reached 83.8% and 64.5%.In solid and liquid conditions,the results indicated that W52 degraded pyrene more effectively than W74.There were obvious differences between the nidA genes coding the large α subunit in the W52 and W74 terminal dioxygenases.We propose that the differences between the two strains abilities to degrade pyrene were related to their terminal dioxygenases." @default.
- W2363396415 created "2016-06-24" @default.
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- W2363396415 date "2008-01-01" @default.
- W2363396415 modified "2023-09-26" @default.
- W2363396415 title "Isolation and identification of two pyrene-degrading mycobacterium sp.strains and their pyrene biodegradation characteristics" @default.
- W2363396415 hasPublicationYear "2008" @default.
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