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- W2363401715 abstract "Objective: To prepare transgenic STO cells of over- expressing GDNF which could be used to enrich spermatogonial stem cells.Methods: GDNF and EGFP bicistronic expression vectors were constructed; the recombinant plasmid was identified by using XhoⅠ,SspⅠ enzyme digestion methods and was transfected into STO cells by using liposome mediated methods. After selection with G418,resistant cells expressing green fluorescence protein were isolated,cultured and expanded; the transgenic cells were identified by PCR. Results: The recombinant GDNF expression vector was successfully constructed and integrated into the cell chromosomes after being transfected into STO cells,GDNF transgenic STO cells expressing green fluorescence protein obtained. Conclusion: Construction of expression vectors and preparation of GDNF transgenic STO cells lay a foundation for the research of enriching spermatogonial stem cells." @default.
- W2363401715 created "2016-06-24" @default.
- W2363401715 creator A5050549448 @default.
- W2363401715 date "2015-01-01" @default.
- W2363401715 modified "2023-09-24" @default.
- W2363401715 title "Construction of the GDNF expression vector and study on the transfected STO cells" @default.
- W2363401715 hasPublicationYear "2015" @default.
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