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- W2363420199 abstract "Objective:Study on extraction of plasmid DNA with phenol-chloroform from clone.Methods:Adopt different extraction times with phenol-chloroform,different acting temperature and acting time of RNaseA.we extracted three kinds of plasmid DNA.Results:extraction twice with phenol-chloroform,acting time of RNaseA was 8 minutes in 45℃.The agarose electrophoresis showed that the quality of plasmid DNA was about OD260/OD280=1.85,and quantity was more than 0.8μg/clone.Conclusion:This method is not only rapid,but also can satisfy the demands of analysis of recombinant plasmids and sequence measure.This method is adapt to sieving of a large amount of clone specially." @default.
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- W2363420199 date "2007-01-01" @default.
- W2363420199 modified "2023-09-28" @default.
- W2363420199 title "A Method of Direct Extraction of Plasmid DNA with Phenol-chloroform from Clone" @default.
- W2363420199 hasPublicationYear "2007" @default.
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