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- W2363516012 abstract "The present sutdy investigated the pathogenicity of velogenic ND viruses of 5 different genotypes in 23-day-old goslings. The challenges of these viruses caused 10%-100% morbidity, and 0%-70% mortality in the infected geese, while the control birds remained healthy. While four NDV field isolates and reference strain of chicken origin which fell into genotype Ⅵg, Ⅶd, Ⅷ and Ⅸ, respectively, caused severe clinical disease in geese, and led to substantial death, similar to those of naturally or experimentally diseased animals infected with isolate of goose origin-genotype Ⅶd; geese inoculated with strain Herts/33 (genotype Ⅳ) and isolate PB9601(Ⅵb) exhibited no overt disease, though depression and diarrhea was observed in very few cases. The localization and distribution of the viral antigen in tissues from the infected goslings were investigated by using immunohistochemical(IHC) staining for detection of NDV F protein. The viral antigen could detected frequently in 17 organs and tissues. Specific staining was localized in the cytoplasma of lymphocytes, reticulocytes, macrophages, hepatocytes as well as various epithelial cells. The positive staining was less intensive in several tissues of goslings infected with Herts/33 and PB9601, compared to that from the same tissue of goslings inoculated with genotype Ⅵg, Ⅶd, Ⅷ and Ⅸ, indicative of the differences of the histotropism of different genotypes of ND viruses. The results of the present study showed that different genotypes of velogenic ND viruses have different pathogenicity to geese." @default.
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- W2363516012 date "2006-06-04" @default.
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- W2363516012 title "Pathogenicity of Different Genotypes of Velogenic Newcastle Disease Virus to Chickens" @default.
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