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- W2363547983 abstract "Objective To study construction and expression of mouse cdc25B protein.Methods The Cdc25B gene was amplified by PCR,enzyme digest and linked behind PGEX4T-2.After inducing with IPTG,the target gene was primarily expressed.Results The Cdc25B gene consisted of about 900bp by DNA electrophoresis.The prokaryotic expression vector of PGEX4T-2/Cdc25B was constructed.After induction,a new anticipated Mr 53KD band appeared in Westen-Blot.Conclusion Fusion proteins of GST/Cdc25B are successfully expressed in BL21.This study is a basis for the role of Cdc25B during mouse oocyte maturatin." @default.
- W2363547983 created "2016-06-24" @default.
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- W2363547983 date "2007-01-01" @default.
- W2363547983 modified "2023-09-24" @default.
- W2363547983 title "Consturction and expression of mouse Cdc25B protein" @default.
- W2363547983 hasPublicationYear "2007" @default.
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