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- W2363562489 abstract "GDNF cDNA holosequence was cloned from rat testis by RT PCR method to clarify the biological activities of GDNF in the nervous system.Sequencing analysis showed that it was completely the same as what reported in GenBank.Then GDNF cDNA was cloned into eucaryotic expression vector pEGFP N1 at 5′ end of EGFP gene encoding green fluorescence protein mutant 1 with human codon usage preferences in the form of nonfused proteins and made it expressed under the control of CMV promoter.The expression of EGFP exhibits that GDNF cDNA can be well expressed in the HeLa cell,a commonly used eucaryotic host cell.To study the effects of GDNF on injured sciatic nerve by plasmid DNA transfection the posthatched chicken of either sex was anesthetized by ether and the right sciatic nerve was transected.Then the mixture of GDNF cDNA recombinant plasmid and lipofectin was injected into the muscles around cut side.The additional injection was performed afeter 5 days.Twenty days later a detecting experiment was carried out.It was observed that the GDNF cDNA transfection could increase 30% motoneuron survivals in lumbar spinal cord(L4-L6) and promote the regeneration of cut sciatic nerves to distal ends." @default.
- W2363562489 created "2016-06-24" @default.
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- W2363562489 date "1999-01-01" @default.
- W2363562489 modified "2023-09-24" @default.
- W2363562489 title "The Cloning of Glial Cell Line derived Neurotrophic Factor cDNA Holosequence and the Studying of Its Biological Activities" @default.
- W2363562489 hasPublicationYear "1999" @default.
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