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- W2363594539 abstract "Objective:Research on the neurotoxicity of glutamate and Aβ.Methods:Cultivate fetal cortex neurons of ICR rats with refined NM-2A medium in 24-well plate on cover glasses at 37℃,5% CO_2 chamber,pretreat cells with glutamate at concentrations of 1mmol/L,5 mmol/L and 10 mmol/L respectivily for 30 min before placing cells into the 5% CO_2 chamber at 37℃ for 24 hours.Treat cells at 37℃ by 20μmol/L Aβ for 24 hours before inspecting the survival rate of cells with MTT or conduct nuclear double staining to analyze the survival rate of cells.Results:Both glutamate and Aβ are toxic to neurons.The toxicive action of glutamate is does-dependent,manifestied as the survival rate of neurons decrease with the rising concentrations of glutamate.Aβ could cause the death of neurons at a concentration of 20μmol/L,with survival rate drops to 69.8% that of control.Conclusion:Glutamate and Aβ can cause necrosis of neurons at certain concentrations." @default.
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- W2363594539 date "2004-01-01" @default.
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- W2363594539 title "The neurotoxicity of glutamate and A#beta# to cortex neurons of mice" @default.
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