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- W2363617009 abstract "The thermal denaturalization was applied to extract superoxide dismutase from porcine blood. On the basis of single-factor experiments, technological paraments were optimized by Box-Behnken central combination design and response surface methodology(RSM) with the specific activity of SOD as index. The crude enzyme was extracted by ethanol-chloroform and acetone, then purified by Sephadex G-100 gel column chromatography.The purified superoxide dismutase was identified by SDS-PAGE. The results showed that the specific activity of enzyme was significantly affected by bath temperature and copper sulfate content. The optimum conditions were bath temperature 66℃, bath time 25min and copper sulfate 3.10%,under which the special activity of crude enzyme was 167.74±0.38 U/mg. After purification, the special activity was increased to 6594.55±16.20 U/mg, with recovery rate of 62.15±0.02%. The purification of the enzyme was analyzed by polyacrylamide gel electrophoresis and the purified enzyme reached electrophoresis level." @default.
- W2363617009 created "2016-06-24" @default.
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- W2363617009 date "2014-01-01" @default.
- W2363617009 modified "2023-09-26" @default.
- W2363617009 title "Extraction of Superoxide Dismutase from Porcine Blood by Response Surface Methodology" @default.
- W2363617009 hasPublicationYear "2014" @default.
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