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- W2363647420 abstract "Objective: To research the proliferation effect of HGF and EGF to the rat fetal liver stem cells at different concentration in vitro.Methods: Using Three-step separation method,fetal liver stem cells were purificated from embryonic liver tissue of pregnant 14 days F344 rats.Cells were treated with condition medium which contains different concentration of HGF and EGF.Cellular proliferation state was observed by electron microscopy.Cellular proliferation of different culture medium was determined by MTT assay.Results: The proliferation effect of HGF 10 ~ 80 ng/ml in each concentration group and EGF 10 ~ 80 ng/ml in each concentration group is greater than that in the control group.As HGFfor20 ng/mL,proliferation effect was significantly greater than control group(P0.01).As HGF concentration continues increasing,no obvious increase of proliferation effect was observed.In 20 ng/mL HGF group combined with different concentration EGF group,we found that in 20 ng/ml HGF with10 ng/mL EGF combination groups,the proliferation effect was increased significantly.As the EGF concentration continues to risen in joint group,the proliferation effect was not improved obviously.Conclusion: HGF and EGF play an important role in improving the serum-free culture conditions of rat fetal liver stem cells.And they have collaborative stimulative effect on rat fetal liver stem cells proliferation in vitro.20 ng/ml HGF combined with 10 ng/mL EGF has significant effect on cellular proliferation of rat fetal liver stem cells." @default.
- W2363647420 created "2016-06-24" @default.
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- W2363647420 date "2013-01-01" @default.
- W2363647420 modified "2023-09-26" @default.
- W2363647420 title "Different Concentrations of Hepatocyte Growth Factor and Epidermal Growth Factor in the Regulation of Fetal Liver Stem Cells in Rats" @default.
- W2363647420 hasPublicationYear "2013" @default.
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