Matches in SemOpenAlex for { <https://semopenalex.org/work/W2363670034> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W2363670034 endingPage "377" @default.
- W2363670034 startingPage "373" @default.
- W2363670034 abstract "OBJECTIVE: To evaluate the expression of dental matrix protein-l (DMP1) in porcine oral mucosa fibroblasts (POMF) transfected by DMP1 and the influences of the transfection. METHODS: The full length of porcine DMP1 cDNA was linked into an eukaryotic expression vector pEGFP-C1. POMF and mesenchymal stem cells (MSC) were transfected with the pEGFP-DMP1. The expression of DMP1, dental sialoprotein (DSP), amelin and ameloblastin (Ambn) gene of transfected POMF and MSC were detected by RT-PCR. The expression of DMP1 and DSP protein was examined by immunocytochemical staining. The formation ratio of mineralized nodules of transfected cells was compared with un-transfected ones after mineralized induction. The formation of mineralized nodules of three-dimensional pellet transfected cells was compared with un-transfected ones after hematoxylin and eosin staining. RESULTS: The constructed pEGFP-DMP1 could produce 4.7 kb and 1.5 kb fragments. DMP1 gene, DSP gene and Ambn gene were expressed by POMF after transfection. Immunohistochemical staining and the quantitative analysis of protein showed that DMP1 and DSP protein was positive in transfected POMF and MSC. The formation ratio of mineralized nodules of transfected POMF and MSC was higher than that of un-transfected ones (P < 0.05). CONCLUSIONS: The expression of porcine DMP1 in POME after gene transfection can induce the expression of tooth-development-associated gene Ambn and DSP and enhance the formation of mineralized nodules." @default.
- W2363670034 created "2016-06-24" @default.
- W2363670034 creator A5016046136 @default.
- W2363670034 creator A5064824633 @default.
- W2363670034 creator A5075877965 @default.
- W2363670034 creator A5080379789 @default.
- W2363670034 creator A5085043657 @default.
- W2363670034 date "2007-06-01" @default.
- W2363670034 modified "2023-09-24" @default.
- W2363670034 title "[An experimental study on porcine fibroblasts transfected by dental matrix protein-1 gene]." @default.
- W2363670034 doi "https://doi.org/10.3760/j.issn:1002-0098.2007.06.016" @default.
- W2363670034 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17888259" @default.
- W2363670034 hasPublicationYear "2007" @default.
- W2363670034 type Work @default.
- W2363670034 sameAs 2363670034 @default.
- W2363670034 citedByCount "0" @default.
- W2363670034 crossrefType "journal-article" @default.
- W2363670034 hasAuthorship W2363670034A5016046136 @default.
- W2363670034 hasAuthorship W2363670034A5064824633 @default.
- W2363670034 hasAuthorship W2363670034A5075877965 @default.
- W2363670034 hasAuthorship W2363670034A5080379789 @default.
- W2363670034 hasAuthorship W2363670034A5085043657 @default.
- W2363670034 hasConcept C104317684 @default.
- W2363670034 hasConcept C150194340 @default.
- W2363670034 hasConcept C153911025 @default.
- W2363670034 hasConcept C185592680 @default.
- W2363670034 hasConcept C2777267240 @default.
- W2363670034 hasConcept C54009773 @default.
- W2363670034 hasConcept C55493867 @default.
- W2363670034 hasConcept C68434959 @default.
- W2363670034 hasConcept C86803240 @default.
- W2363670034 hasConceptScore W2363670034C104317684 @default.
- W2363670034 hasConceptScore W2363670034C150194340 @default.
- W2363670034 hasConceptScore W2363670034C153911025 @default.
- W2363670034 hasConceptScore W2363670034C185592680 @default.
- W2363670034 hasConceptScore W2363670034C2777267240 @default.
- W2363670034 hasConceptScore W2363670034C54009773 @default.
- W2363670034 hasConceptScore W2363670034C55493867 @default.
- W2363670034 hasConceptScore W2363670034C68434959 @default.
- W2363670034 hasConceptScore W2363670034C86803240 @default.
- W2363670034 hasIssue "6" @default.
- W2363670034 hasLocation W23636700341 @default.
- W2363670034 hasLocation W23636700342 @default.
- W2363670034 hasOpenAccess W2363670034 @default.
- W2363670034 hasPrimaryLocation W23636700341 @default.
- W2363670034 hasRelatedWork W2353030114 @default.
- W2363670034 hasRelatedWork W2357152280 @default.
- W2363670034 hasRelatedWork W2358491215 @default.
- W2363670034 hasRelatedWork W2360160741 @default.
- W2363670034 hasRelatedWork W2361596128 @default.
- W2363670034 hasRelatedWork W2371737301 @default.
- W2363670034 hasRelatedWork W2372922059 @default.
- W2363670034 hasRelatedWork W2375091408 @default.
- W2363670034 hasRelatedWork W2375596216 @default.
- W2363670034 hasRelatedWork W2377022585 @default.
- W2363670034 hasRelatedWork W2378391410 @default.
- W2363670034 hasRelatedWork W2378405106 @default.
- W2363670034 hasRelatedWork W2386181594 @default.
- W2363670034 hasRelatedWork W2387594040 @default.
- W2363670034 hasRelatedWork W2393485552 @default.
- W2363670034 hasRelatedWork W2419346850 @default.
- W2363670034 hasRelatedWork W2438883477 @default.
- W2363670034 hasRelatedWork W3033376363 @default.
- W2363670034 hasRelatedWork W3149126048 @default.
- W2363670034 hasRelatedWork W99273658 @default.
- W2363670034 hasVolume "42" @default.
- W2363670034 isParatext "false" @default.
- W2363670034 isRetracted "false" @default.
- W2363670034 magId "2363670034" @default.
- W2363670034 workType "article" @default.