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- W2363673842 abstract "The conditions for protoplast preparation in the fusion of inactivated parental protoplasts of Aspergillus oryzae were studied in the article.The pretreatment of mycelium and the enzymatic hydrolysis of cell wall were combined into one stage.The addition amount of DDT was discussed.The results showed that the optimal enzyme for cell wall broken in protoplast preparation were the mixture of cellulase,lywallzyme and snailase,which was mixed at 5:3:1.The mycelium culture time was 15h.DDT was added at 3 mmol/L during 2.5 h hydrolysis,in which 0.8 mol/L NaCl was used as osmotic stabilizers.The fusion rate of the inactivated parental protoplasts was 3.31%." @default.
- W2363673842 created "2016-06-24" @default.
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- W2363673842 date "2007-01-01" @default.
- W2363673842 modified "2023-09-24" @default.
- W2363673842 title "Protoplast preparation in fusion of inactivated parental protoplasts of Aspergillus oryzae" @default.
- W2363673842 hasPublicationYear "2007" @default.
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