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- W2363683013 abstract "Objective:To develop a quantitative determination of puerarin,daidzin,genistin,daidzein in the fraction extracted of pueraria isoflavone.Methods:The samples were analyzed on a Kromasil KR100-5 C18(4.6 mm×250 mm,5 μm) column with acetonitrile-0.03 mol·L-1 ammonium acetate as a mobile phase using a gradient elution.The flow rate was 0.7 mL·min-1,and UV detection wavelength was set at 250 nm.Results:The linear ranges of puerarin,daidzin,genistin,daidzein were 0.13-0.66 μg,0.03-0.14 μg,0.03-0.16 μg,0.02-0.12 μg,respectively(r﹥0.9995).The average recoveries were 98.1%-99.3%.Conclusion:The new method is fast,accurate,sensitive,selective,and suitable for use in the quality control of pueraria isoflavone fraction." @default.
- W2363683013 created "2016-06-24" @default.
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- W2363683013 date "2009-01-01" @default.
- W2363683013 modified "2023-09-23" @default.
- W2363683013 title "RP-HPLC simultaneous determination the content of puerarin,daidzin,genistin,daidzein in the fraction extracted of pueraria isoflavone" @default.
- W2363683013 hasPublicationYear "2009" @default.
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