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- W2363689267 abstract "The expression of α 1,3 Gal in inbred strain Wuzhishan miniature porcine was determined by flow cytometry and confocal analysis.The porcine peripheral blood mononuclear cells(PBMCs) were isolated from heparinised whole blood of 5-month old porcine by density gradient centrifugation and then incubated with FITC-isolectin.The specificity of FITC-isolectin binding to α 1,3 Gal was tested in porcine kidney epithelial PK15 and human HEK293 cells.No detectable fluorescence was observed in HEK293 cells with 100 ng/μl FITC-isolectin treatment,while the fluorescence was detected in PK15 cells with only 1.560 ng/μl FITC-isolectin treatment.The optimal concentration of FITC-isolectin was determined as 25 ng/μl(labeling rate85%) for PK15 cells.The samples were analyzed on a Calibur FACScan and BX51 Laser scanning confocal microscope.The α 1,3 Gal levels were found to display a 1.25～2.09 folds variation in the samples examined,indicating the variation of α 1,3 Gal expression between individual porcine appeared to be limited.These findings may further validate the inbred Wuzhishan miniature porcines as the candidate organ donors for xenotransplantation." @default.
- W2363689267 created "2016-06-24" @default.
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- W2363689267 date "2010-01-01" @default.
- W2363689267 modified "2023-09-23" @default.
- W2363689267 title "Variations Analysis of α1,3 Gal Level in Wuzhishan Miniature Porcine" @default.
- W2363689267 hasPublicationYear "2010" @default.
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