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- W2363693704 abstract "A rice cDNA fragment c73, one of the candidates for the sequence encoding a DNA binding protein, has been isolated from a rice cDNA library, using a 31 bp DNA fragment in the 5' upstream region of rice waxy gene as a probe. To isolate and purify the gene product, the c73 DNA was cloned into a His 6 fusion expression vector pET28 c(+) (Fig.1). After being transformed into E.coli BL21(DE3), the expected fusion protein encoded by c73 fragment was expressed by induction by IPTG, and purified using Ni NTA affinity chromatography. Under the optimized experimental conditions (Fig.2), the expressed fusion protein was predominantly located in E.coli cells in soluble form, without accumulating as inclusion bodies. This protein constituted about 10% of the total bacterial proteins (Fig.3); and can be purified in one step to a higher than 90% purity from bacterial lysates using Ni NTA resin (Fig.4)." @default.
- W2363693704 created "2016-06-24" @default.
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- W2363693704 date "1999-01-01" @default.
- W2363693704 modified "2023-09-25" @default.
- W2363693704 title "Expression of a Rice cDNA c73 in E.coli Cells and Purification of Its Fusion Protein" @default.
- W2363693704 hasPublicationYear "1999" @default.
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