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- W2363701521 abstract "Objective To investigate the difference between two EBV negative nasopharyngeal carcinoma(NPC) cell lines,namely,C666-1 and CNE2,in 5-ALA-PDT efficacy in different cell growth phases. Methods The cell growth curves for EBV-C666-1 and CNE2 cells were developed by cell counting.Laser-induced fluorescence technique and confocal microscope were used to study the accumulated concentration and subcellular localization of 5-ALA-PpⅨ in C666-1 and CNE2 cells respectively.The survival rates of the two NPC cell lines were analyzed with CCK-8. Results The PpⅨ production of CNE2 cells was higher than that of C666-1 cells,and correlated with the growth phases of the cells.After 4 hours' incubation with 5-ALA,the induced PpⅨ in C666-1 cells was found mainly in mitochondria,whereas it concentrated in cytomembrane for CNE2 cells.After the PDT treatment,the survival rate of C666-1 cells was lower than that of CNE2 cells both for 24 and 48 hours' cultivation respectively,but the rate for 72 hours' cultivation was higher. Conclusions The difference between C666-1 and CNE2 cells in PpⅨ production during different growth phases was significant,and the efficacy of 5-ALA-PDT depends on both the concentration and subcellular distribution of 5-ALA induced PpⅨ." @default.
- W2363701521 created "2016-06-24" @default.
- W2363701521 creator A5026904351 @default.
- W2363701521 date "2013-01-01" @default.
- W2363701521 modified "2023-09-23" @default.
- W2363701521 title "Efficacy of 5-ALA-PDT in Killing EBV-C666-1 and CNE2 Cells" @default.
- W2363701521 hasPublicationYear "2013" @default.
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