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- W2363715907 abstract "Immature embryos and embryogenic calli of wheat( Triticum aestivian L.) cultivars Jinanl77, 99P and Hesheng 3 were infected by three types of Agrobacterium LBA4404/pCDH, Agll/pUNN2 and AglO without plasmid. Transgenic plants regenerated only from embryogenic calli. PCR and PCR-Southern hybridization confirmed that transgenic plants contained foreign genes. Chromosomes of embryogenic calli infected were analysis. The elimination and breakage were more severe in the calli infected by LBA4404 than by AglO or control without infected.The factors impacted on transformation efficiency were also researched, including Agrobacterium types, wheat genotypes,explant types and period of selection etc." @default.
- W2363715907 created "2016-06-24" @default.
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- W2363715907 date "2004-01-01" @default.
- W2363715907 modified "2023-09-23" @default.
- W2363715907 title "Agrobacterium tumefaciens mediated choline dehydrogenase gene(betA) transfer to wheat and factors impacting on transformation" @default.
- W2363715907 hasPublicationYear "2004" @default.
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