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- W2363748067 abstract "OBJECTIVETo isolate and purify functional Torpedo acetylcholine receptor (tAChR). METHODSThe muscle of electronic organ of Torpedo californica was homogenized, and from which the crude extracts of tAChR were isolated, and subsequently, purified through cobra-toxin coupled Sepharose 4B chromatography.The binding activity of the purified tAChR to anti-tAChR antibody was determined using enzyme-linked immunosorbent assay (ELISA).RESULTSThe purified tAChR was able to bind to anti-tAChR antibody with high binding activity.CONCLUSIONThe isolated and purified AChR from Torpedo electronic organ is of high activity and of specificity, and can be used as antigen to examine anti-acetylcholine receptor antibodies in ELISA." @default.
- W2363748067 created "2016-06-24" @default.
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- W2363748067 date "2004-01-01" @default.
- W2363748067 modified "2023-09-28" @default.
- W2363748067 title "Isolation and purification of Torpedo acetylcholine receptor" @default.
- W2363748067 hasPublicationYear "2004" @default.
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