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- W2363762425 abstract "Objective:to research the use of PCR in detecting and diagnosing ecninococsus granulosus and conduct a diagnostic technique by DNA hybridization labeled with DIG.Methods:A pairs of primer were designed according to echinococcus granulosus gene fragment clone and sequence analysis special primer as:P1 5'-GGAATGGAGAGAAGTTAC-3',P2 5'-GCAACCTCCGGAACTTGC-3'.The hydatid fluid,secondary hydatid and protoscolex of echinococcus granulosus were used as template,417bp special band was got after being amplified by PCR.The PCR product was purified and labeled with DIG.The special DNA probe was succefully got and can be used to detect the echinococcus granulosus.Result:The DNA from colibacillus;Shigella,tubercle,Cysticercus granulosus and health human leukocyte were extended by PCR and hybrided by DNA probe labeled with DIG,only cystilercus granulosus express 471bp special band.The sensitivity of PCR were that one cystilercus granulosus can be detected or 100-10 fg DNA.while the dot hybridization was 2500fg.Heterologous DNA will not express positive reaction,even increasing the spotting membrane dosages.Conclusions:The sequence of PCR labeled with DIG showed good specificity,high sensitivity,more accuracy and quickness and so it can provide scientific basis to early diagnosis echinococcus granulosus and its epidemiological investigation." @default.
- W2363762425 created "2016-06-24" @default.
- W2363762425 creator A5052140744 @default.
- W2363762425 date "2002-01-01" @default.
- W2363762425 modified "2023-09-25" @default.
- W2363762425 title "Detection of Echiococcosis by PCR and the Establishment of Diagnosis Technique by DNA Hybridization Labeled With DIG" @default.
- W2363762425 hasPublicationYear "2002" @default.
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