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- W2363762912 abstract "Objective To investigate the influence of recombinant adenovirus-mediated heat shock protein 70(HSP70) on growth of neuronal cells damaged by hypoxia-reoxygenation.Methods Recombinant adenovirus vAd-HSP70 carrying total length HSP70 gene were used to infect nerve cells primarily cultured in vitro,the expression of exogenous HSP70 in target cells was detected by RT-PCR and Western blotting.The experiment was carried out in three groups: vAd-HSP70-infected group,vAd-GFP-infected group,and non-infected group.After treatment with hypoxia-reoxygenation in the three groups,MTT was used to detect the cell viability;growth curve to detect the cell growth;ELISA to detect the lactic dehydrogenase(LDH) activity in supernatant of cell culture.Results The expression of HSP70 gene was identified in the vAd-HSP70-infected group by RT-PCR and Western blotting.After hypoxia-reoxygenation treatment,the cell viability in vAd-HSP70-infected group was significantly higher than that of the other two groups(F=10.683,P0.01).The growth curve showed the cells in vAd-HSP70-infected group grow well,the cell number was much more than that in the other two groups(F=21.307-24.105,P0.01);the LDH viability in vAd-HSP70-infected group was significantly lower than that in the other two groups(F=7.492,P0.01),and the difference of LDH viability between vAd-GFP-infected and non-infected groups was not significant(P0.05).Conclusion Recombinant adenovirus-mediated heat shock protein 70 can protect the growth of nerve cells,thus resist hypoxia-reoxygenation injury." @default.
- W2363762912 created "2016-06-24" @default.
- W2363762912 creator A5070546544 @default.
- W2363762912 date "2011-01-01" @default.
- W2363762912 modified "2023-09-23" @default.
- W2363762912 title "INFLUENCE OF RECOMBINANT HEAT SHOCK PROTEIN 70 ADENOVIRUS ON GROWTH OF NEURONAL CELLS AGAINST HYPOXIA-REOXYGENATION" @default.
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