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- W2363772022 abstract "Objective To study the DNA damage of Chinese hamster lung(CHL) fibroblas cell line induced by Rehmanniae Radix extract using single cell gel electrophoresis(SCGE)(comet assay) so as to provide the reference for preclinical safety evaluation of the drug.Methods Taking H2O2 as positive drug,the cells were treated with Rehmanniae Radix extract at the concentrations of 0.2,1.0,and 5.0 mg/mL,respectively for 24 h,then the comet assays were performed.Results The obvious damage of CHL cells was induced by H2O2 and the significant difference was indicated by the the average tail length of comet in compared with the negative control group.There was no significant difference of every index,such as comet length and comet tail length at various concentrations of Rehmanniae Radix extract.Conclusion The comet assay shows considerable sensitivity to detect the lesion process.Different concentrations of Rehmanniae Radix extract could not induce DNA damage to CHL cells." @default.
- W2363772022 created "2016-06-24" @default.
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- W2363772022 date "2011-01-01" @default.
- W2363772022 modified "2023-09-22" @default.
- W2363772022 title "Analysis on DNA damage of Chinese hamster lung fibroblas cell line induced by Rehmanniae Radix extract with single cell gel electrophoresis" @default.
- W2363772022 hasPublicationYear "2011" @default.
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