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- W2363775231 abstract "Serum albumin of Monopterus albus was obtained through the steps of mixing ion-exchange and gel filtration chromatography, and named MAA-serum, which is a single chain protein with molecular weigh of 67 kDa. MAA-serum is not a glycoprotein. The N-terminal amino acid sequence is GHKKW. The purified MAA-serum was used to immunize rabbits to prepare polyclonal antibody with the dosage of 0.5 mg/per rabbit. After immunized three times, the rabbits were anesthetized with ether, then the blood was collected by a cardiac puncture, and precipitate with saturated ammonium sulfate to obtain polyclonal antibody. ELISA data demonstrated that the antibody titer of the serum was up to 1∶100 000. The anti MAA-serum polyclonal antibody had a high quality of potency and specificity. The antibody provided an useful experimental tool to profoundly research the tissue expression profile and biological function of MAA-serum." @default.
- W2363775231 created "2016-06-24" @default.
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- W2363775231 date "2012-01-01" @default.
- W2363775231 modified "2023-09-26" @default.
- W2363775231 title "Purification and antibody preparation of serum albumin from Monopterus albus" @default.
- W2363775231 hasPublicationYear "2012" @default.
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