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- W2363776314 abstract "Objective To investigate the gene expression profile of the dorsal hippocampus following transient global ischemia in rats.Methods The rat models of global ischemia were established.The total RNAs of sham-operated group and ischemia group were extracted from the dorsal hippocampus after ischemia for 6 and 24 h.All of the RNAs were purified and converted to cDNAs by reverse transcription.The cDNAs were labeled with Cy5(ischemia group) or Cy3(sham-operated group) and hybridized to a rat whole-genome oligonucleotide microarray.The gene expression profiles were analyzed using bioinformatics methods.Results A total of 491 genes,including 351 genes at 6 h and 296 genes at 24 h after ishemcia,were up-regulated.A total of 387 genes,including 296 genes at 6 h and 179 genes at 24 h after ischemia,were down-regulated.And then,these differentially-expressed genes were analyzed using Cluster analysis and KEGG pathway analysis.Conclusions Hundreds of differentially-expressed genes were found by analyzing the whole-genome oligonucleotide microarray combined with the bioinformatics approaches.This result provides some useful information for clarifying the molecular mechanism of brain ischemia injury." @default.
- W2363776314 created "2016-06-24" @default.
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- W2363776314 date "2011-01-01" @default.
- W2363776314 modified "2023-09-26" @default.
- W2363776314 title "Genome-wide gene expression analysis of the dorsal hippocampus following transient global ischemia in rats" @default.
- W2363776314 hasPublicationYear "2011" @default.
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