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- W2363792523 abstract "[Objective]To investigate the effect of 17β-estradiol(E2) on m RNA expression levels of the β-defensin-2(SBD-2)in ovine oviduct epithelial cells in vitro. [Method]The primary ovine oviduct epithelial cells were isolated and subcultured to 2nd passage. The 2ndpassage cells were divided into five groups and were treated with 10-6mol/L, 10-7mol/L, 10-8mol/L, 10-9mol/L and 10-10mol/L of E2, respectively. The m RNA expression levels of the β-defensin-2(SBD-2) of ovine oviduct epithelial cells were determined at 2thh, 6thh, 12 thh, 24 thh and 48 thh after they were treated with different concentration of E2 by real-time RTPCR, respectively. The blank control groups were also included in each E2 treated groups for each detection time. [Results] The m RNA expression of SBD-2 of ovine oviduct epithelial cells reached the extreme level when they were treated with E2 of 10-8mol/L for 6 h and was significantly upregulated compared with the control(P0.01), and the m RNA expression of the SBD-2 of the cells were also upregulated when they were treated with E2 of 10-10mol/L for 24 h and 48 h. The expression of the SBD-2 of the cells showed a gradual decrement trend with the increase of E2 concentration. [Conclusion]It was indicated that the m RNA expression of SBD-2 of ovine oviduct epithelial cells can be upregulated by specific concentration of E2 for certain time, and the effect of E2 on the expression of the SBD-2 of ovine oviduct epithelial cells showed a time effect and dose-dependent manner." @default.
- W2363792523 created "2016-06-24" @default.
- W2363792523 creator A5043698782 @default.
- W2363792523 date "2015-01-01" @default.
- W2363792523 modified "2023-09-23" @default.
- W2363792523 title "Effects of 17β-estradiol on β-defensin-2(SBD-2) Expression in the Ovine Oviduct Epithelial Cells" @default.
- W2363792523 hasPublicationYear "2015" @default.
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