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- W2363811925 abstract "Triplo-lethal Locus(Tpl) was surveyed as the only one that both triplo-lethal and haplo-lethal gene locus in the Drosophila genome.There was an Osiris gene family named gene Osiris1 through Osiris20 in the Tpl region.Through BLASTTP and PSI-BLAST analysis,there were only three other members of this family located outside of this locus.We called them Osiris21 to Osiris23.This gene family had the Insecta specificity.Through bioinformatics,RT-PCR and RACE methods,six silkworm genes belonging to Osiris gene family have been cloned and named as BmOsiris7,BmOsiris9,BmOsiris18,BmOsiris19,BmOsiris20 and BmOsiris21,respectively.Sequence analysis indicated that BmOsiris gene′s ORF were between 728 bp and 882 bp in length.The molecular size of deduced amino acid which seemed quite similar with Drosophila Osiris protein and were 26-31 kDa range.The analysis of isoelectric point indicated that BmOsiris21′s isoelectric point was 9.0,others were at between 6.41 and 8.66.SMART conservative region prediction on line found that this protein family belong to transmembrane protein and had specific conservative regions for the Osiris family,called DUF1676.All above dated supported the speculation that the cloned genes belonged to Osiris gene family.Phylogenetic tree analysis demonstrated paralogs of Bombyx mori and Drosophila melanogaster were much closer to the infraspecific orthologs.Expression pattern analysis indicated that each gene in our tested samples had no tissue and space-time speciality,but expression amount had significant differences.chromosome location showed that BmOsiris7、BmOsiris9、 BmOsiris18、BmOsiris19 and BmOsiris20 located on the Chr.26,however,BmOsiris21 individually located on Chr.4.This result was very similar with Osiris genes from Drosophila melanogaster." @default.
- W2363811925 created "2016-06-24" @default.
- W2363811925 creator A5076493506 @default.
- W2363811925 date "2010-01-01" @default.
- W2363811925 modified "2023-09-26" @default.
- W2363811925 title "Cloning,Sequence Structure and Expression Analysis of Osiris Gene Family in the Silkworm,Bombyx mori" @default.
- W2363811925 hasPublicationYear "2010" @default.
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