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- W2363822346 abstract "Objective To investigate the effects of sodium hyaluronate solution on the osteoplastic differentiation of rat's myoblasts.Methods The minced muscles were digested with mixed digestive juices,followed by the isolation,culture and purification of the myoblasts.The third subculture myoblasts were cultured in two growth media,in which experimental group containing 0.1% sodium hyaluronate and 500 ng/mL rhBMP-2,the control group containing 500 ng/mL rhBMP-2,respectively.The morphological change of induced myoblasts in each medium was observed carefully.Three weeks later,a series of examination were performed,including:ALP activity,typeⅠcollagen immunohistochemistry,and new calcifying nodules staining with Alizarin red S and ALP.Results The morphological change in each medium was similar during the induced process.There was a difference between the two groups on ALP activity,but the ALP activity increased and there was no statistic difference(P 0.05).The immunohistochemistry of typeⅠcollagen was positive,a great quantity of new calcifying nodules was seen by the staining of Alizarin red S and ALP.Conclusion Sodium hyaluronate could not inhibit the osteoplastic differentiation of rat's myoblasts in vitro,it could be a proper media for myoblasts." @default.
- W2363822346 created "2016-06-24" @default.
- W2363822346 creator A5087611833 @default.
- W2363822346 date "2011-01-01" @default.
- W2363822346 modified "2023-09-23" @default.
- W2363822346 title "Effect of Sodium Hyaluronate on In Vitro Osteoplastic Differentiation of Rat's Myoblasts" @default.
- W2363822346 hasPublicationYear "2011" @default.
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