Matches in SemOpenAlex for { <https://semopenalex.org/work/W2363826377> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2363826377 abstract "Objective To study the influence of different blood serum concentration and in vitro growth time on the curve and morphological character of in vitro cultured L929 cell line of mouse fibroblasts.Methods L929 cell line of the mouse fibroblasts were cultured by in vitro cell culture technology,and the sulforhodamine B and microplate reader(with a wavelength of under 490 nm) were also performed to measure absorbency of every hole and transform it into growth curve of L929 cell line of the fibroblasts.Meanwhile,staining methods of HE,acridine orange fluorescence and Hoechst33342 fluorescence were respectively used to observe the morphology of L929 cell line of mouse fibroblasts.Results L929 cell line of the mouse fibroblasts,when cultured in blood serum of different concentrations for different time(2,4,6,8 d),displayed different growth rates,survival rates and cell morphology.When cultured for the same time in blood serum of different concentrations(0%,20%,40%,60%,80%,and 100%),the results were as follows: when the concentration were 20% and 40%,OD values were tended to increase rapidly and the cell growth were promoted strongly;when the concentrations were 0% and 100%,OD values were tended to decrease steadily and the cell growth was inhibited obviously.When cultured in the same serum for different time,OD values in other groups were tended to increase with time except 0% group.OD values were increased rapidly within the first 4 days,then the increase was slowed down from 4 to 6 days and the tendency of increase was recovered from 6 to 8 days,particularly for 100% group.In this case,morphology of the mouse fibroblasts L929 cells was also changed.Conclusions Different serum concentrations and different growth time in vitro affect cell growth,survival and morphology of mouse L929 fibroblast cell line significantly.Growth time and the blood serum concentration should be fully considered when the cell is cultured in an in vitro condition.This finding provides some reference value for future cytological studies in skin anti-aging." @default.
- W2363826377 created "2016-06-24" @default.
- W2363826377 creator A5015105956 @default.
- W2363826377 date "2011-01-01" @default.
- W2363826377 modified "2023-09-25" @default.
- W2363826377 title "The influence of different blood serum concentration and growth time upon the growth curve and the morphological character of in vitro cultured L929 cell line of mouse fibroblasts" @default.
- W2363826377 hasPublicationYear "2011" @default.
- W2363826377 type Work @default.
- W2363826377 sameAs 2363826377 @default.
- W2363826377 citedByCount "0" @default.
- W2363826377 crossrefType "journal-article" @default.
- W2363826377 hasAuthorship W2363826377A5015105956 @default.
- W2363826377 hasConcept C109316439 @default.
- W2363826377 hasConcept C1491633281 @default.
- W2363826377 hasConcept C149782125 @default.
- W2363826377 hasConcept C153911025 @default.
- W2363826377 hasConcept C162324750 @default.
- W2363826377 hasConcept C16685009 @default.
- W2363826377 hasConcept C185592680 @default.
- W2363826377 hasConcept C202751555 @default.
- W2363826377 hasConcept C2583947 @default.
- W2363826377 hasConcept C2776074239 @default.
- W2363826377 hasConcept C2776913854 @default.
- W2363826377 hasConcept C2779102032 @default.
- W2363826377 hasConcept C29537977 @default.
- W2363826377 hasConcept C54355233 @default.
- W2363826377 hasConcept C55493867 @default.
- W2363826377 hasConcept C62112901 @default.
- W2363826377 hasConcept C71924100 @default.
- W2363826377 hasConcept C74864618 @default.
- W2363826377 hasConcept C81885089 @default.
- W2363826377 hasConcept C86803240 @default.
- W2363826377 hasConceptScore W2363826377C109316439 @default.
- W2363826377 hasConceptScore W2363826377C1491633281 @default.
- W2363826377 hasConceptScore W2363826377C149782125 @default.
- W2363826377 hasConceptScore W2363826377C153911025 @default.
- W2363826377 hasConceptScore W2363826377C162324750 @default.
- W2363826377 hasConceptScore W2363826377C16685009 @default.
- W2363826377 hasConceptScore W2363826377C185592680 @default.
- W2363826377 hasConceptScore W2363826377C202751555 @default.
- W2363826377 hasConceptScore W2363826377C2583947 @default.
- W2363826377 hasConceptScore W2363826377C2776074239 @default.
- W2363826377 hasConceptScore W2363826377C2776913854 @default.
- W2363826377 hasConceptScore W2363826377C2779102032 @default.
- W2363826377 hasConceptScore W2363826377C29537977 @default.
- W2363826377 hasConceptScore W2363826377C54355233 @default.
- W2363826377 hasConceptScore W2363826377C55493867 @default.
- W2363826377 hasConceptScore W2363826377C62112901 @default.
- W2363826377 hasConceptScore W2363826377C71924100 @default.
- W2363826377 hasConceptScore W2363826377C74864618 @default.
- W2363826377 hasConceptScore W2363826377C81885089 @default.
- W2363826377 hasConceptScore W2363826377C86803240 @default.
- W2363826377 hasLocation W23638263771 @default.
- W2363826377 hasOpenAccess W2363826377 @default.
- W2363826377 hasPrimaryLocation W23638263771 @default.
- W2363826377 hasRelatedWork W147433397 @default.
- W2363826377 hasRelatedWork W1509254016 @default.
- W2363826377 hasRelatedWork W1975797622 @default.
- W2363826377 hasRelatedWork W1986325639 @default.
- W2363826377 hasRelatedWork W1987652313 @default.
- W2363826377 hasRelatedWork W2004401603 @default.
- W2363826377 hasRelatedWork W2068255034 @default.
- W2363826377 hasRelatedWork W2071680924 @default.
- W2363826377 hasRelatedWork W2074412740 @default.
- W2363826377 hasRelatedWork W2157369174 @default.
- W2363826377 hasRelatedWork W2297438102 @default.
- W2363826377 hasRelatedWork W2398890690 @default.
- W2363826377 hasRelatedWork W2402209618 @default.
- W2363826377 hasRelatedWork W2411359362 @default.
- W2363826377 hasRelatedWork W2412627224 @default.
- W2363826377 hasRelatedWork W2415368252 @default.
- W2363826377 hasRelatedWork W2442124495 @default.
- W2363826377 hasRelatedWork W2615421449 @default.
- W2363826377 hasRelatedWork W2767367976 @default.
- W2363826377 hasRelatedWork W2419218926 @default.
- W2363826377 isParatext "false" @default.
- W2363826377 isRetracted "false" @default.
- W2363826377 magId "2363826377" @default.
- W2363826377 workType "article" @default.