Matches in SemOpenAlex for { <https://semopenalex.org/work/W2363837104> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2363837104 abstract "Objective To establish HER-2 mRNA detection system by reverse transcription quantitative real-time PCR(RT-qPCR) for archival paraffin-embedded breast cancer tissue.Methods Paraffin was removed by xylene followed by extracting total RNA with FFPE RNA extraction kit.After reverse transcription to cDNA,HER-2,ACTB and GAPDH were detected by specific primers and hydrolysis probe.The normal expression unit of HER-2 was calculated by optimized △CT algorithm method,and the results of RT-qPCR were compared to those of fluorescence in situ hybridization(FISH) for consistency.Results In this study,the concordance between RT-qPCR and FISH methods was 95.7%.Conclusion With improved experimental design and quality control,paraffin-embedded breast cancer tissues are the ideal material for detecting HER-2 mRNA expression." @default.
- W2363837104 created "2016-06-24" @default.
- W2363837104 creator A5025617080 @default.
- W2363837104 date "2012-01-01" @default.
- W2363837104 modified "2023-09-28" @default.
- W2363837104 title "Establishment of HER-2 mRNA quantitative measurement system for archival paraffin-embedded breast cancer tissue" @default.
- W2363837104 hasPublicationYear "2012" @default.
- W2363837104 type Work @default.
- W2363837104 sameAs 2363837104 @default.
- W2363837104 citedByCount "0" @default.
- W2363837104 crossrefType "journal-article" @default.
- W2363837104 hasAuthorship W2363837104A5025617080 @default.
- W2363837104 hasConcept C104317684 @default.
- W2363837104 hasConcept C105580179 @default.
- W2363837104 hasConcept C112190416 @default.
- W2363837104 hasConcept C121608353 @default.
- W2363837104 hasConcept C153911025 @default.
- W2363837104 hasConcept C156719811 @default.
- W2363837104 hasConcept C187882448 @default.
- W2363837104 hasConcept C4692481 @default.
- W2363837104 hasConcept C48023723 @default.
- W2363837104 hasConcept C530470458 @default.
- W2363837104 hasConcept C54355233 @default.
- W2363837104 hasConcept C55493867 @default.
- W2363837104 hasConcept C67705224 @default.
- W2363837104 hasConcept C81439078 @default.
- W2363837104 hasConcept C86803240 @default.
- W2363837104 hasConceptScore W2363837104C104317684 @default.
- W2363837104 hasConceptScore W2363837104C105580179 @default.
- W2363837104 hasConceptScore W2363837104C112190416 @default.
- W2363837104 hasConceptScore W2363837104C121608353 @default.
- W2363837104 hasConceptScore W2363837104C153911025 @default.
- W2363837104 hasConceptScore W2363837104C156719811 @default.
- W2363837104 hasConceptScore W2363837104C187882448 @default.
- W2363837104 hasConceptScore W2363837104C4692481 @default.
- W2363837104 hasConceptScore W2363837104C48023723 @default.
- W2363837104 hasConceptScore W2363837104C530470458 @default.
- W2363837104 hasConceptScore W2363837104C54355233 @default.
- W2363837104 hasConceptScore W2363837104C55493867 @default.
- W2363837104 hasConceptScore W2363837104C67705224 @default.
- W2363837104 hasConceptScore W2363837104C81439078 @default.
- W2363837104 hasConceptScore W2363837104C86803240 @default.
- W2363837104 hasLocation W23638371041 @default.
- W2363837104 hasOpenAccess W2363837104 @default.
- W2363837104 hasPrimaryLocation W23638371041 @default.
- W2363837104 hasRelatedWork W2018264742 @default.
- W2363837104 hasRelatedWork W2043886895 @default.
- W2363837104 hasRelatedWork W2062385231 @default.
- W2363837104 hasRelatedWork W2080959094 @default.
- W2363837104 hasRelatedWork W2087382663 @default.
- W2363837104 hasRelatedWork W2092109743 @default.
- W2363837104 hasRelatedWork W2164629904 @default.
- W2363837104 hasRelatedWork W2349902826 @default.
- W2363837104 hasRelatedWork W2350044114 @default.
- W2363837104 hasRelatedWork W2368726071 @default.
- W2363837104 hasRelatedWork W2368847918 @default.
- W2363837104 hasRelatedWork W2374807797 @default.
- W2363837104 hasRelatedWork W2379243234 @default.
- W2363837104 hasRelatedWork W2471843704 @default.
- W2363837104 hasRelatedWork W2522526970 @default.
- W2363837104 hasRelatedWork W2599482806 @default.
- W2363837104 hasRelatedWork W2616902355 @default.
- W2363837104 hasRelatedWork W2753558691 @default.
- W2363837104 hasRelatedWork W3149298283 @default.
- W2363837104 hasRelatedWork W2044150990 @default.
- W2363837104 isParatext "false" @default.
- W2363837104 isRetracted "false" @default.
- W2363837104 magId "2363837104" @default.
- W2363837104 workType "article" @default.