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- W2363837269 abstract "Objective:To study the conditions of in vitro proliferation and differentiation of rat bone marrow stromal cells(MSC) for its application in transplantation.Methods:MSC were obtained by flushing the shaft with DMEM using a sterile syringe,dissociated by density gradient centrifuge,and cultured in the medium containing DMEM and 10% fatal bovine serum(FBS).Then we added neural growth factors into the medium and observed their influence on MSC proliferation,differentiation and survival by phase contrast microscopy.Immunocytochemistry was used to identify cell types.Results:MSC cultured in medium containing DMEM/ 10% FBS/ neural growth factors proliferated quickly.The neuron-like cells appeared at 7-9 d with the expression of neurone specific enolase(NSE) at(52±9.2)% and glial fibrillary acidic protein(GFAP) at(15±7)%,and could survive more than 7 days;In contrast,cells induced by β-mercaptoethanol(BME) died gradually after 24 hours.Conclusion:MSC can not only proliferate and differentiate into neuron-like cells effectively in the medium containing neural growth factors but also survive longer." @default.
- W2363837269 created "2016-06-24" @default.
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- W2363837269 date "2007-01-01" @default.
- W2363837269 modified "2023-09-26" @default.
- W2363837269 title "Experimental study of rat bone marrow stromal cell proliferation and differentiation in vitro" @default.
- W2363837269 hasPublicationYear "2007" @default.
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