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- W2363847073 abstract "Objective To establish the method of in vitro culture and expansion of adult human bone marrow-derived mesenchymal stem cells (MSCs) and to investigate their biological characteristics.Methods Adult human MSCs cultured in Dulbecco's Modified Eagle's Medium with low glucose containing 10% fetal bovine serum. The FACS was performed to examine the expression of cell surface molecules and DNA content. Results The MSCs yielded in this study were homogenous population and had multiplication potential. Over (2～3)×10 8 MSCs would be obtained from each 10-ml bone marrow aspirate at 5th passage. The cells were positive for CD29、CD44、CD166, and negative for CD14、CD34、CD45、HLA-DR.Conclusion The method can isolate and culture a homogenous MSC population that have unique growth phenotype." @default.
- W2363847073 created "2016-06-24" @default.
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- W2363847073 date "2003-01-01" @default.
- W2363847073 modified "2023-09-24" @default.
- W2363847073 title "The in vitro expansion and biological characteristics of adult human bone marrow-derived mesenchymal stem cells" @default.
- W2363847073 hasPublicationYear "2003" @default.
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