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- W2363861282 abstract "Objective To investigate the effect of transfection recombinant sense and antisense α smooth muscle actin (α SMA) gene on fibroblasts from the scar and skin retrovirus. Methods A recombinant sense and antisense α SMA cDNA was obtained using gene recombinant technique to clone the α SMA cDNA to vector, and then transfected into cultured fibroblasts from the scar and skin. The cellular morphology and gene expression of sense and antisense α SMA in them were observed by immunofluorescent method. Results The recombinant antisense α SMA retrovirus inhibited fibroblast proliferation and reduced the volumes of cells. The expression of α SMA in the fibroblasts decreased in a time dependent manner. However, the recombinant sense α SMA retrovirus had an opposite function to that of antisense α SMA. Conclusion Recombinant sense and antisense α SMA retrovirus can affect the morphology, growth and cell function of the fibroblasts from the hypertrophic scar and skin." @default.
- W2363861282 created "2016-06-24" @default.
- W2363861282 creator A5032378175 @default.
- W2363861282 date "2003-01-01" @default.
- W2363861282 modified "2023-09-25" @default.
- W2363861282 title "Effect of recombinant sense and antisense α-SMA retroviral vector on fibroblasts isolated from scar tissue and skin" @default.
- W2363861282 hasPublicationYear "2003" @default.
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