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- W2363886101 abstract "Objective To construct and screen shRNA plasmids of HSPA8 with high interfering ability and provide the material for further researching on the effect of HSPA8 gene on breast cancer.Methods In accordance with the sequence of HSPA8 gene in GenBank,four interfering gene fragments and four shRNA interfering plasmids(pGPU6/GFP/Neo-349,pGPU6/GFP/Neo-818,pGPU6/GFP/Neo-931,pGPU6/GFP/Neo-1180)were constructed.The plasmids were transfected into human breast cancer cell MCF-7 respectively,and negative control group and blank control group were set up.The HSPA8 positive expressing cells were screened by G418.The interfering efficiencies of the plasmids on HSPA8 gene were detected at the mRNA and protein levels by RT-PCR and Western blotting.Results The RT-PCR results showed that at the mRNA level in four transfected groups the expression levels were decreased by 25.0%,37.5%,43.7%,and 68.5%,respectively,compared with untransfected group.The Western blotting results showed that the differences of the protein expression levels between transfected groups and blank control group were significant(P0.05).The interfering efficiency of pGPU6/GFP/Neo-1180 plasmid was the highest.Conclusion The high ability shRNA interfering plasmids of HSPA8 gene are constructed successfully.The pGPU6/GFP/Neo-1180 interfering plasmid may provide a basis for further study on the occurrence and development of breast cancer." @default.
- W2363886101 created "2016-06-24" @default.
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- W2363886101 date "2010-01-01" @default.
- W2363886101 modified "2023-09-24" @default.
- W2363886101 title "Effect of RNA interfering on expression of HSPA8 gene in MCF-7 cells" @default.
- W2363886101 hasPublicationYear "2010" @default.
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