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- W2363972013 abstract "Objective o investigate the effect of batroxobin(DF-521)on the number and the activity of endothelial progenitor cells (EPCs) in vitro and its possible mechanism. Methods The mononuclear cells were collected from the human peripheral blood,isolated by the density gradient centrifugation and cultured. On the 6th day, the phenotypes(CD34,CD31,VEGF-2, vWF) of the attached cells were assayed by flow cytometry(FCM). The cells were observed by the laser scanning confocal microscope after double staining with FITC-UEA-I and DiI-acLDL. After identified, the EPCs were cultured in three kinds of nutrient liquid which included different doses of DF-521. The phenotypes(CD31 ,vWF) of EPCs were assessed on the 14th and 21st day respectively by FCM. The proliferation and angiogenesis of EPCs in vitro were analyzed by cell counting and tube formation. NO concentrations were measured with the nitrate reductase method. The ultrastructure of the cells was observed with a transmission electron microscope(TEM). Results The number,expression of CD31 and vWF and the NO concentration in EPCs were remarkably increased after treatment with DF-521 compared with those in the control (P0.05). Furthermore, these effects had dose-dependent manner (P0.05). In the high dose group, the EPCs formed shuttle-like and tube-like structures after being plated in matrigel. The W-P bodies could be found in the cells by the TEM. Conclusion The proliferation,angiogenesis.differentiation into endothelial cells and the activity of excreting NO of EPCs can be improved by DF-521 in vitro. The endothelial nitric oxide synthase may play a key role in these effects." @default.
- W2363972013 created "2016-06-24" @default.
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- W2363972013 date "2009-01-01" @default.
- W2363972013 modified "2023-09-23" @default.
- W2363972013 title "Effect of batroxobin on the activity of endothelial progenitor cells and its possible mechanism in vitro" @default.
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