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- W2363973270 abstract "[Objective] In order to appraise the utility of 16S rRNA gene for the differentiation of different bacte- ria, a pair of primers were selected to amplify this gene. The PCR products were then digested by restriction en- donuclease. [Method] All the bacteria were lysed by heat and amplified by two kinds of fluorescence -FAM and JOE, respectively, labeled primers. The PCR product were digested by Hae III and then taken capillary electrophoresis. [Result] All the bacteria can generated a 255bp product after amplification. The products di- gested were different in different families. [Conclusion] This method can be applied to the practice of differenti- ation of different bacteria belonging to different families." @default.
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- W2363973270 date "2005-01-01" @default.
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- W2363973270 title "Bacterial families classification by RFLP" @default.
- W2363973270 hasPublicationYear "2005" @default.
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