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- W2363974178 abstract "Objective To develop a biotin-streptavidin-enzyme-linked immunosorbent assay (BSA-ELISA) for detection of human cardiotrophin-1 (CT-1) in plasma and investigate its preliminary application in the patients with coronary heart disease.Methods The main steps of the BSA-ELISA were as follows:96 well plates were coated with specific mouse IgG1 antibodies to human CT-1.After adding CT-1 standards or plasma samples,biotinylated specific goat IgG antibodies to human CT-1 were used as the second antibody.Streptavidin-HRP-IgG solution was then added and the colour was revealed with OPD.According to the standard curves,concentration of CT-1 in sample was determined.The plasma CT-1 levels in 35 patients with coronary heart disease and controls were measured.Results The working range of standard curve was 10～2 000 pg/ml,and the minimum detectable limit of the assay was 10 pg/ml.Variance coefficient within group was 1.01%～7.64% and variance coefficient between groups was 5.96%～9.04%.Conclusion The BSA-ELISA for plasma CT-1 developed in this study is a specific,sensitive and convenient method which should be suitable for clinical measurement and basal research." @default.
- W2363974178 created "2016-06-24" @default.
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- W2363974178 date "2005-01-01" @default.
- W2363974178 modified "2023-09-26" @default.
- W2363974178 title "A biotin-streptavidin-enzyme-linked immunosorbent assay for detection of human cardiotrophin-1" @default.
- W2363974178 hasPublicationYear "2005" @default.
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