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- W2363982557 abstract "Objective To establish a novel co-cultures model of suprachiasmatic nucleus(SCN) slices and NIH/3T3 cells in a serum-free condition.Methods SCN or cortical slices prepared from 7-day-old male SD rats were cultured with NIH/3T3 cells for 6 d.24 h expression profile of Per1 was examined in NIH/3T3 cells using real-time PCR.Results A significant daily oscillation of Per1 mRNA expression was observed in NIH/3T3 cells co-cultured with SCN slices.The peak time of Per1 was at CT11.No daily oscillation was found in NIH/3T3 cells induced with cortical slices.Conclusion A novel co-culture model of SCN slices and NIH/3T3 cells was established,which will facilitate the studies on the communications between SCN and peripheral tissues." @default.
- W2363982557 created "2016-06-24" @default.
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- W2363982557 date "2009-01-01" @default.
- W2363982557 modified "2023-09-23" @default.
- W2363982557 title "Establishment of a Novel Co-culture Model of Suprachiasmatic Nucleus Slices and NIH/3T3 Cells in a Serum-free Condition" @default.
- W2363982557 hasPublicationYear "2009" @default.
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